Yayın:
Radiation-induced formation of polyacrylic acid-protein covalent conjugates

Basit öğe sayfası
dc.contributor.authorMustafaev, M
dc.contributor.authorBayülken, S
dc.contributor.authorErgen, E
dc.contributor.authorErkol, AY
dc.contributor.authorArdagil, N
dc.date.accessioned2026-06-27T12:59:31Z
dc.date.issued2001
dc.description.abstractRadiation-induced formation of water-soluble covalent conjugates of polyacrylic acid (PAA) with negatively charged proteins - bovine serum albumin (BSA) and superoxide dismutase (SOD)- was investigated at neutral pH. Aqueous solutions of PAA, BSA, SOD and their mixtures (PAA-BSA, PAA-Cu2+-BSA, PAA-SOD) have been irradiated with Co-60 gamma -rays and the resulting changes have been measured by high-performance liquid chromatography and UV-Visible spectrophotometry. Crosslinking and formation of water-soluble conjugates of PAA with BSA molecules upon irradiation were observed in the PAA-BSA and PAA-Cu2+ -BSA mixtures. In contrast to these systems, Cu(II), Zn(II) ions in the structure of SOD molecules protect the free protein, and the protein component of PAA-SOD mixtures against radiation damage, and prevent the PAA-SOD covalent conjugate formation. The mechanism of observed protection effect is discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.en
dc.description.urihttps://doi.org/10.1016/s0969-806x(00)00423-0
dc.identifier.doi10.1016/s0969-806x(00)00423-0
dc.identifier.endpage575
dc.identifier.issn0969-806X
dc.identifier.issue6
dc.identifier.startpage567
dc.identifier.urihttps://hdl.handle.net/20.500.14981/48668
dc.identifier.volume60
dc.identifier.wos000168046000003
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofRADIATION PHYSICS AND CHEMISTRY
dc.subjectBOVINE SUPEROXIDE DISMUTASE
dc.subjectSERUM-ALBUMIN
dc.subjectCOMPLEXES
dc.subjectWATER
dc.subjectCU2+
dc.subjectChemistry
dc.subjectNuclear Science & Technology
dc.subjectPhysics
dc.titleRadiation-induced formation of polyacrylic acid-protein covalent conjugates
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

Dosyalar

Koleksiyonlar

Yayınlar