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Noncovalent Immobilization of Pectinesterase (Prunus armeniaca L.) onto Bentonite

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dc.contributor.authorKarakus, Emine
dc.contributor.authorOzler, Aynur
dc.contributor.authorPekyardimci, Sule
dc.date.accessioned2026-06-27T13:07:54Z
dc.date.issued2008
dc.description.abstractIn this work, pectinesterase isolated from Malatya apricot was immobilized onto acid-treated bentonite surface by simple adsorption at pH 9.0. The properties of free and immobilized enzyme were defined. The effect of various factors such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. Optimum pH and temperature were determined to be 9.0 and 50 degrees C, respectively. Kinetic parameters of the immobilized enzyme ( Km and Vmax values) were also determined as 0.51 mM of the Km and 14.6 mu mol min(-1) mg(-1) of the Vmax. No drastic change was observed in the Km value after immobilization. The Vmax value of immobilized enzyme was 8.4-fold bigger than those of free enzyme. Thermal and storage stability experiments were carried out. The patterns of heat stability indicated that the immobilization process tends to stabilize the enzyme. The properties of the immobilized enzyme were compared to those of the free enzyme.en
dc.description.sponsorshipState Planning Organization (DPT) Research Fund [98 K - 120830]
dc.description.urihttps://doi.org/10.1080/10731190802554455
dc.identifier.doi10.1080/10731190802554455
dc.identifier.eissn1532-4184
dc.identifier.endpage550
dc.identifier.issn1073-1199
dc.identifier.issue6
dc.identifier.pubmed19058058
dc.identifier.startpage535
dc.identifier.urihttps://hdl.handle.net/20.500.14981/50161
dc.identifier.volume36
dc.identifier.wos000261848000006
dc.language.isoeng
dc.publisherINFORMA HEALTHCARE
dc.relation.ispartofARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
dc.rightsopenAccess
dc.subjectbentonite
dc.subjectpectinesterase
dc.subjectpurification
dc.subjectnon-covalent immobilization
dc.subjectadsorption
dc.subjectiocemical characterization
dc.subjectPECTIN METHYLESTERASE
dc.subjectTOMATO PECTINESTERASE
dc.subjectCANDIDA-RUGOSA
dc.subjectBRAMLEY APPLE
dc.subjectLIPASE
dc.subjectENZYME
dc.subjectFRUIT
dc.subjectINACTIVATION
dc.subjectEXTRACTION
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering
dc.subjectMaterials Science
dc.titleNoncovalent Immobilization of Pectinesterase (Prunus armeniaca L.) onto Bentonite
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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