In-situ formation of self-assembled Ag nanoclusters on ct-DNA in the presence of 2-mercaptothioxanthone by using UV-vis light irradiation

Abstract

Self-assembled silver nanoparticles (Ag NPs) on calf thymus DNA (ct-DNA) chains were synthesized in the presence of 2-mercaptothioxanthone (TX-SH) by use of UV irradiation technique. Thioxanthone itself and its derivatives show interesting photochemical and biological properties. The formation of Ag NPs on the ct-DNA was achieved with and without TX-SH as a thioxanthone derivative photoinitiator which played a photoreducing role for the formation of Ag NPs. The photoreducing reaction of Ag ions to Ag NPs was followed by UV-vis absorption spectroscopy. A new band was observed at 428 nm which was a good sign of the surface plasmon resonance (SPR) of Ag NPs. Fluorescence quenching was effectively observed depending on the irradiation time of the solution, possibly as a result of Ag NPs size. Particle size analysis and Scanning Electron Microscopy (SEM) imaging confirmed the size of the formed Ag NPs and silver nanoclusters (Ag NCs) effectively. In this study, we report for the first time the formation of self-assembled Ag NPs on ct-DNA in the presence of 2-mercaptothioxanthone by photoirradiation technique. TX-SH played two important roles; photoreducing and photostabilizing agent for the formation of Ag NCs on ct-DNA. Fluorophore character of synthesized Ag NPs on DNA may help to develop new techniques for biological and medical aspects. (C) 2017 Elsevier B.V. All rights reserved.