Yayın:
Enhanced Stability and Decolorization of Coomassie Brilliant Blue R-250 by Dextran Aldehyde-modified Horseradish Peroxidase

dc.contributor.authorAltikatoglu, Melda
dc.contributor.authorCelebi, Mithat
dc.date.accessioned2026-06-27T13:14:59Z
dc.date.issued2011
dc.description.abstractHorseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.en
dc.description.urihttps://doi.org/10.3109/10731199.2010.533124
dc.identifier.doi10.3109/10731199.2010.533124
dc.identifier.eissn1532-4184
dc.identifier.endpage190
dc.identifier.issn1073-1199
dc.identifier.issue3
dc.identifier.pubmed21117874
dc.identifier.startpage185
dc.identifier.urihttps://hdl.handle.net/20.500.14981/51031
dc.identifier.volume39
dc.identifier.wos000290672200012
dc.language.isoeng
dc.publisherINFORMA HEALTHCARE
dc.relation.ispartofARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
dc.subjectHRP
dc.subjectdextran aldehyde
dc.subjectmodification
dc.subjectCoomassie Brilliant Blue R-250
dc.subjectdecolorization
dc.subjectAZO-DYE
dc.subjectINTERFACIAL INACTIVATION
dc.subjectTEXTILE
dc.subjectDEGRADATION
dc.subjectREMOVAL
dc.subjectOXIDASE
dc.subjectIMMOBILIZATION
dc.subjectNANOPARTICLES
dc.subjectTEMPERATURE
dc.subjectEFFLUENT
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering
dc.subjectMaterials Science
dc.titleEnhanced Stability and Decolorization of Coomassie Brilliant Blue R-250 by Dextran Aldehyde-modified Horseradish Peroxidase
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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