Yayın: Enhanced Stability and Decolorization of Coomassie Brilliant Blue R-250 by Dextran Aldehyde-modified Horseradish Peroxidase
| dc.contributor.author | Altikatoglu, Melda | |
| dc.contributor.author | Celebi, Mithat | |
| dc.date.accessioned | 2026-06-27T13:14:59Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP. | en |
| dc.description.uri | https://doi.org/10.3109/10731199.2010.533124 | |
| dc.identifier.doi | 10.3109/10731199.2010.533124 | |
| dc.identifier.eissn | 1532-4184 | |
| dc.identifier.endpage | 190 | |
| dc.identifier.issn | 1073-1199 | |
| dc.identifier.issue | 3 | |
| dc.identifier.pubmed | 21117874 | |
| dc.identifier.startpage | 185 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14981/51031 | |
| dc.identifier.volume | 39 | |
| dc.identifier.wos | 000290672200012 | |
| dc.language.iso | eng | |
| dc.publisher | INFORMA HEALTHCARE | |
| dc.relation.ispartof | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | |
| dc.subject | HRP | |
| dc.subject | dextran aldehyde | |
| dc.subject | modification | |
| dc.subject | Coomassie Brilliant Blue R-250 | |
| dc.subject | decolorization | |
| dc.subject | AZO-DYE | |
| dc.subject | INTERFACIAL INACTIVATION | |
| dc.subject | TEXTILE | |
| dc.subject | DEGRADATION | |
| dc.subject | REMOVAL | |
| dc.subject | OXIDASE | |
| dc.subject | IMMOBILIZATION | |
| dc.subject | NANOPARTICLES | |
| dc.subject | TEMPERATURE | |
| dc.subject | EFFLUENT | |
| dc.subject | Biotechnology & Applied Microbiology | |
| dc.subject | Engineering | |
| dc.subject | Materials Science | |
| dc.title | Enhanced Stability and Decolorization of Coomassie Brilliant Blue R-250 by Dextran Aldehyde-modified Horseradish Peroxidase | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.import.source | WOS |