Synthesis of 4-thiazolidinone derivatives and assessment of their toxicological properties

Abstract

The current study was designated to assess the potential toxicological effects of six newly synthesized 4-thiazolidinone derivatives by using Ames-Salmonella, Escherichia coli WP2, Zea mays seed germination and random amplified polymorphic DNA (RAPD) assay systems. The bacterial tester strains S. typhimurium TA1535, TA1537, TA1538, TA98, TA100 and E. coli WP2uvrA were chosen to check the gene mutation causing capabilities of the test materials in prokaryotic systems and Z. mays seeds for determination of toxicological potentials in eukaryotic systems. Furthermore, OPA-4 and OPY-13 primers were used in RAPD analysis to determine genotoxic activities on the eukaryotic genomes. According to the results, none of the test materials showed significant mutagenic activity on the bacterial tester strains at tested concentrations. On the other hand, all of them showed significant inhibition on the germination of Z. mays seeds in a dose dependent manner from 2 mM to 6 mM concentrations. The germination was completely inhibited at 6 mM and higher dose levels. The RAPD analysis results also showed a parallelism with the germination assay results and the test materials significantly changed the RAPD profiles for both primers when compared with the control groups. In a conclusion, these newly synthesized 4-thiazolidinone derivatives were determined as genotoxically suspect compounds according to the presented results, and they should analyze with multiple toxicology test systems before performing other types of biological activity determination studies.