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Immobilization of glutaminase enzyme from Hypocria jecorina on polyacrylic acid: preparation and biochemical characterization

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dc.contributor.authorKarahan, Mesut
dc.contributor.authorKarakus, Emine
dc.contributor.authorBulbul, Dilara
dc.contributor.authorAtaci, Nese
dc.date.accessioned2026-06-27T13:30:16Z
dc.date.issued2014
dc.description.abstractL-glutaminase enzyme produced from Hypocrea jecorina pure culture and polyacrylic acid (PAA) in the presence Cu2+ ions were composed the ternary complex at pH 7. The properties of free and immobilized enzyme were defined. The effect of various factors such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. The properties of immobilized enzyme were investigated and compared to those of free enzyme. Optimum pH and temperature of both enzyme were determined to be 8.0 and 50 degrees C, respectively. Kinetic parameters of the immobilized enzyme (Km and Vmax values) were also determined as 0.38 mM of the Km and 10.9 U/L of the Vmax. No drastic change was observed in the Km and Vmax values. Thermal and storage stability experiments were carried out. The thermal stability studies indicated that the immobilization process tends to stabilize the enzyme.en
dc.description.sponsorshipYildiz Technical University Science Research Projects Foundation [2011-01-02-KAP07]
dc.description.urihttps://doi.org/10.3109/21691401.2013.808646
dc.identifier.doi10.3109/21691401.2013.808646
dc.identifier.eissn2169-141X
dc.identifier.endpage267
dc.identifier.issn2169-1401
dc.identifier.issue4
dc.identifier.pubmed23802704
dc.identifier.startpage262
dc.identifier.urihttps://hdl.handle.net/20.500.14981/53302
dc.identifier.volume42
dc.identifier.wos000339802500006
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
dc.subjectenzyme production
dc.subjectL-Glutaminase enzyme
dc.subjectimmobilization
dc.subjectmicroorganism
dc.subjectpolyacrylic acid
dc.subjectternary metal complexes
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectSALT-TOLERANT
dc.subjectAMINO-ACIDS
dc.subjectASPARAGINASE
dc.subjectBIOSENSOR
dc.subjectSURFACE
dc.subjectOXIDASE
dc.subjectBEADS
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering
dc.subjectMaterials Science
dc.titleImmobilization of glutaminase enzyme from Hypocria jecorina on polyacrylic acid: preparation and biochemical characterization
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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