Yayın:
Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus

Basit öğe sayfası
dc.contributor.authorOzkan, Busra
dc.contributor.authorBudama-Kilinc, Yasemin
dc.contributor.authorCakir-Koc, Rabia
dc.contributor.authorMes, Sevim
dc.contributor.authorBadur, Selim
dc.date.accessioned2026-06-27T14:21:55Z
dc.date.issued2019
dc.description.abstractThe diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method.en
dc.description.sponsorshipScientific and Technological Research Council of Turkey [115S132]
dc.description.urihttps://doi.org/10.1080/21655979.2019.1586054
dc.identifier.doi10.1080/21655979.2019.1586054
dc.identifier.eissn2165-5987
dc.identifier.endpage42
dc.identifier.issn2165-5979
dc.identifier.issue1
dc.identifier.pubmed30913952
dc.identifier.startpage33
dc.identifier.urihttps://hdl.handle.net/20.500.14981/59749
dc.identifier.volume10
dc.identifier.wos000462377000001
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofBIOENGINEERED
dc.rightsopenAccess
dc.subjectImmunoglobulin Y
dc.subjectinfluenza A
dc.subjectalkaline phosphatase
dc.subjectELISA methods
dc.subjectdiagnosis
dc.subjectLINKED-IMMUNOSORBENT-ASSAY
dc.subjectMONOCLONAL-ANTIBODY
dc.subjectMATRIX PROTEIN-2
dc.subjectM2 PROTEIN
dc.subjectVACCINE
dc.subjectGENERATION
dc.subjectECTODOMAIN
dc.subjectFORM
dc.subjectIGY
dc.subjectBiotechnology & Applied Microbiology
dc.titleApplication of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

Dosyalar

Koleksiyonlar

Yayınlar