Determination of homocystine as dabsyl derivative by HPLC using precolumn derivatization

Abstract

An HPLC method has been developed for the determination of homocystine. Homocystine was converted to its chromophoric dabsyl derivative and was chromatographed by a reversed phase chromatographic column (mu-Bondapak C) with pH = 3 phosphate buffer-acetonitrile (60:40) as isocratic mobile phase. The derivative was detected by a variable wavelength detector operating at 436 ran. A linear relationship was obtained between peak areas and homocystine concentrations over the range of 0.54-2.68 mu g / 20 mu l.