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SHAPE Analysis of Small RNAs and Riboswitches

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dc.contributor.authorRice, Greggory M.
dc.contributor.authorBusan, Steven
dc.contributor.authorKarabiber, Fethullah
dc.contributor.authorFavorov, Oleg V.
dc.contributor.authorWeeks, Kevin M.
dc.date.accessioned2026-06-27T13:31:19Z
dc.date.issued2014
dc.description.abstractWe describe structural analysis of small RNAs by SHAPE chemical probing. RNAs are treated with 1-methyl-7-nitroisatoic anhydride, a reagent that detects local nucleotide flexibility; and N-methylisatoic anhydride and 1-methyl-6-nitroisatoic anhydride, reagents which together detect higher-order and noncanonical interactions. Chemical adducts are quantified as stops during reverse transcriptase-mediated primer extension. Probing information can be used to infer conformational changes and ligand binding and to develop highly accurate models of RNA secondary structures.en
dc.description.sponsorshipNIGMS NIH HHS [R01 GM098662] Funding Source: Medline
dc.description.sponsorshipDiv Of Molecular and Cellular Bioscience
dc.description.sponsorshipDirect For Biological Sciences [1121024] Funding Source: National Science Foundation
dc.description.urihttps://doi.org/10.1016/b978-0-12-801122-5.00008-8
dc.identifier.doi10.1016/b978-0-12-801122-5.00008-8
dc.identifier.endpage187
dc.identifier.isbn978-0-12-801122-5
dc.identifier.issn0076-6879
dc.identifier.pubmed25432749
dc.identifier.startpage165
dc.identifier.urihttps://hdl.handle.net/20.500.14981/53506
dc.identifier.volume549
dc.identifier.wos000381763600009
dc.language.isoeng
dc.publisherELSEVIER ACADEMIC PRESS INC
dc.relation.ispartofRIBOSWITCH DISCOVERY, STRUCTURE AND FUNCTION
dc.subjectSELECTIVE 2'-HYDROXYL ACYLATION
dc.subjectSECONDARY
dc.subjectACCURATE
dc.subjectREAGENT
dc.subjectBiochemistry & Molecular Biology
dc.titleSHAPE Analysis of Small RNAs and Riboswitches
dc.typeReview; Book Chapter
dspace.entity.typePublication
local.import.sourceWOS

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