Yayın:
Comparison of Covalent and Noncovalent Immobilization of Malatya Apricot Pectinesterase (Prunus armeniaca L.)

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dc.contributor.authorKarakus, Emine
dc.contributor.authorPekyardimci, Sule
dc.date.accessioned2026-06-27T13:17:34Z
dc.date.issued2012
dc.description.abstractPectinesterase isolated from Malatya apricot pulp was noncovalently and covalently immobilized onto bentonite and glutaraldehyde-containing amino group functionalized porous glass beads surface at pH 8.0 and pH 9.0, respectively. The effect of various parameters such as pH, temperature, activation energy, heat and storage stability on immobilized enzyme were investigated. The optimum temperature of covalently and noncovalently immobilized PE was 50 degrees C. This value was 60 degrees C for free PE. Although optimum pH of covalently-immobilized PE was 8.0, this parameter was 9.0 for free and covalently-immobilized PE. The noncovalently immobilized enzyme exhibited better thermostability than the free and covalently immobilized PE.en
dc.description.sponsorshipState Planning Organization (DPT) Research Fund [98 K - 120830]
dc.description.urihttps://doi.org/10.3109/10731199.2011.611471
dc.identifier.doi10.3109/10731199.2011.611471
dc.identifier.eissn1532-4184
dc.identifier.endpage141
dc.identifier.issn1073-1199
dc.identifier.issue1-2
dc.identifier.pubmed21951194
dc.identifier.startpage132
dc.identifier.urihttps://hdl.handle.net/20.500.14981/51553
dc.identifier.volume40
dc.identifier.wos000299696500017
dc.language.isoeng
dc.publisherINFORMA HEALTHCARE
dc.relation.ispartofARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
dc.rightsopenAccess
dc.subjectPectinesterase
dc.subjectPurification
dc.subjectBentonite
dc.subjectAdsorption
dc.subjectGlass beads
dc.subjectEnzyme immobilization
dc.subjectNon-covalent immobilization
dc.subjectCovalent immobilization
dc.subjectCharacterization
dc.subjectPECTIN METHYL ESTERASE
dc.subjectPOLYPHENOL OXIDASE
dc.subjectMETHYLESTERASE
dc.subjectFRUIT
dc.subjectPECTINMETHYLESTERASE
dc.subjectINACTIVATION
dc.subjectEXTRACTION
dc.subjectSURFACES
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering
dc.subjectMaterials Science
dc.titleComparison of Covalent and Noncovalent Immobilization of Malatya Apricot Pectinesterase (Prunus armeniaca L.)
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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