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A potentiometric biosensor for the determination of valproic acid: Human blood-based study of an anti-epileptic drug

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dc.contributor.authorOzbek, Oguz
dc.contributor.authorIsildak, Omer
dc.contributor.authorIsildak, Ibrahim
dc.date.accessioned2026-06-27T14:38:11Z
dc.date.issued2021
dc.description.abstractA potentiometric poly (vinyl chloride) (PVC) membrane-based biosensor has been developed for the determination of the anti-epileptic drug valproic acid in human blood samples. The valpmate-selective biosensor worked well over a wide concentration range from 1.0 x 10(-6) to 1.0 x 10(-1) mol L-1 with Nernstian slope of 59.0 + 3.6 mV/decade. The biosensor exhibited a lower detection limit of 9.75 x 10(-7) mol L-1. The developed valproate-selective biosensor showed very good selectivity for anti-epileptic drugs such as carbamazepine, levetiracetam and phenytoin, and for some ions present in human blood. The biosensor displayed a fast response time of less than 10 s, good reusability and stability, and operated in the wide pH range of 4.0-11.0. The morphology of the developed PVC membrane biosensor was analyzed by scanning electron microscopy. Finally, the determination of valproic acid in human blood samples was carried out using the standard addition method with very high recoveries.en
dc.description.sponsorshipTokat Gaziosmanpasa University, Scientific Research Projects Commission [2019/89]
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)-BIDEB 2211/A
dc.description.sponsorshipCouncil of Higher Education (YOK) 100/2000 National Scholarship Programs for PhD students
dc.description.sponsorshipTUBITAK
dc.description.sponsorshipYOK
dc.description.urihttps://doi.org/10.1016/j.bej.2021.108181
dc.identifier.doi10.1016/j.bej.2021.108181
dc.identifier.eissn1873-295X
dc.identifier.issn1369-703X
dc.identifier.urihttps://hdl.handle.net/20.500.14981/62935
dc.identifier.volume176
dc.identifier.wos000697108600019
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofBIOCHEMICAL ENGINEERING JOURNAL
dc.rightsopenAccess
dc.subjectValproic acid
dc.subjectEpilepsy
dc.subjectBiosensor
dc.subjectAnti-epileptic
dc.subjectPotentiometry
dc.subjectION-SELECTIVE ELECTRODES
dc.subjectSURFACE-CHARGE DENSITY
dc.subjectSODIUM VALPROATE
dc.subjectPVC MEMBRANE
dc.subjectSENSOR
dc.subjectPHARMACOKINETICS
dc.subjectCONSTRUCTION
dc.subjectMETABOLITES
dc.subjectSERUM
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering
dc.titleA potentiometric biosensor for the determination of valproic acid: Human blood-based study of an anti-epileptic drug
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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