Yayın:
HE EFFECT OF HORSERADISH PEROXIDASE (HRP)-DEXTRAN CONJUGATE ON NAPHTOL BLUE BLACK

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dc.contributor.authorOnder, R. Selva
dc.contributor.authorCelebi, Mithat
dc.contributor.authorAltikatoglu, Melda
dc.contributor.authorKuzu, Huriye
dc.date.accessioned2026-06-27T13:06:29Z
dc.date.issued2009
dc.description.abstractThe removal of azo dye Naphtol Blue Black in aqueous media with the catalysis of HRP-dextran conjugate was studied. HRP was purified by affinity chromatography using immobilized Con A. Enzyme-dextran conjugate with 1/10 molar ratio was synthesized using dextran aldehyde derivative (Dextran Mw 75.000 Da) and purified enzyme. The reaction was carried out at pH 3, 4, 5, 6, 7 and 8 in a 25, 30, 35, 40, 45, 50, 60, 70 degrees C temperatured crystal cuvette of UV-Vis spectrophotometer. The bleaching of the dye depending on the time was observed with the absorption spectra recorded regularly for one hour. The best results were obtained at pH 4 and 5 for all temperatures studied.en
dc.identifier.eissn1304-7191
dc.identifier.endpage25
dc.identifier.issn1304-7205
dc.identifier.issue1
dc.identifier.startpage18
dc.identifier.urihttps://hdl.handle.net/20.500.14981/49822
dc.identifier.volume27
dc.identifier.wos000219484500002
dc.language.isotur
dc.publisherYILDIZ TECHNICAL UNIV
dc.relation.ispartofSIGMA JOURNAL OF ENGINEERING AND NATURAL SCIENCES-SIGMA MUHENDISLIK VE FEN BILIMLERI DERGISI
dc.subjectHRP
dc.subjectdextran
dc.subjectconjugate
dc.subjectnaphtol blue black
dc.subjectdye bleaching
dc.subjectDEGRADATION
dc.subjectENZYMES
dc.subjectDYES
dc.subjectPURIFICATION
dc.subjectWATER
dc.subjectEngineering
dc.titleHE EFFECT OF HORSERADISH PEROXIDASE (HRP)-DEXTRAN CONJUGATE ON NAPHTOL BLUE BLACK
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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