Yayın:
Characterization of Water-Soluble Complexes of Polyacrylic Acid with α-Amylase from Aspergillus oryzae

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dc.contributor.authorAltikatoglu, Melda
dc.contributor.authorTavukcuoglu, Oezlem
dc.contributor.authorMustafaev, Mamed
dc.date.accessioned2026-06-27T12:51:55Z
dc.date.issued2010
dc.description.abstractPolycomplex formation of alpha-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. Acording to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI). The stability of PAA-amylase complexes was negligibly weak at pH 7 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes were formed at pH 5 (pI similar to 4.5) and coexisted with free protein molecules. Insoluble complexes has been observed at pH < 4.5. The frozen storage stabilities of the obtained complexes were also studied by measuring the activities at different pH.en
dc.description.urihttps://doi.org/10.1007/s10930-010-9231-y
dc.identifier.doi10.1007/s10930-010-9231-y
dc.identifier.endpage126
dc.identifier.issn1572-3887
dc.identifier.issue2
dc.identifier.pubmed20165908
dc.identifier.startpage120
dc.identifier.urihttps://hdl.handle.net/20.500.14981/47604
dc.identifier.volume29
dc.identifier.wos000275462900008
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofPROTEIN JOURNAL
dc.subjectPolyelectrolytes
dc.subjectEnzyme
dc.subjectHPLC
dc.subjectFluorescence
dc.subjectStabilization
dc.subjectBOVINE SERUM-ALBUMIN
dc.subjectPOLY(ACRYLIC ACID)
dc.subjectTRYPTOPHAN
dc.subjectBINDING
dc.subjectRESOLUTION
dc.subjectBiochemistry & Molecular Biology
dc.titleCharacterization of Water-Soluble Complexes of Polyacrylic Acid with α-Amylase from Aspergillus oryzae
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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