SYNTHESIS AND PHYSICOCHEMICAL CHARACTERIZATION OF COVALENT CONJUGATES: SHEEP-PDX DISEASE'S SYNTHETIC PEPTIDE POLYACRYLIC ACID

Abstract

The aim of this study was to synthesize water soluble conjugates with PAA polymer and synthetic Sheep-pox viruses' 92-118 peptide series which belongs to P32 major antigen. The synthesis of conjugates was performed with various concentrations of the components (n=1, 3, 5, 10, 15, 20). The synthesized conjugates were characterized with Gel Permeation Chromatography (GPC), ZetaSizer and fluorescence spectroscopy. Following the analyses, the effect of n=15 conjugate on L929 mice fibroblast cells was investigated comparative with free peptide and free polymer and its non-toxic concentrations on the cell were established. The obtained results showed that; the conjugates were synthesized with high yield, all of the peptide molecules have participated in conjugation formation, the size of the conjugate changes depending on peptide and polymer concentration and the fluorescence intensity decreases in every conjugation ratio. The results indicate the conjugation reaction was performed successfully and the n=15 conjugate was biocompatible in every concentration studied.