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A rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES

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dc.contributor.authorBodur, Suleyman
dc.contributor.authorErarpat, Sezin
dc.contributor.authorBalcik, Utku
dc.contributor.authorBakirdere, Sezgin
dc.date.accessioned2026-06-27T14:33:05Z
dc.date.issued2021
dc.description.abstractThis study proposed a novel analytical method for the separation and determination of cobalamin and cobalt in kefir samples by high performance liquid chromatography-inductively coupled plasma-optical emission spectrometry (HPLC-ICP-OES) in addition to determination of cobalamin in HPLC system. Chromatographic parameters such as column type, buffer solution, mobile phase flow rate and sample injection volume were individually studied and optimized. In addition, cobalamin was simultaneously determined by high performance liquid chromatography with ultraviolet detection (HPLC-UV). LOD values of cobalt in cobalamin and cobalt for HPLC-ICP-OES system were calculated as 0.07 mg/kg (as Co) and 0.06 mg/kg, respectively. Recovery studies were conducted to evaluate the accuracy/applicability of the method. Recovery results for cobalt in cobalamin and cobalt detected by the HPLC-ICP-OES system were calculated in the range of 87.4-100.1 and 98.8-115.0%, respectively while recovery results for cobalamin were found to be between 89.2 and 98.3% for HPLC-UV system.en
dc.description.urihttps://doi.org/10.1016/j.foodchem.2020.127945
dc.identifier.doi10.1016/j.foodchem.2020.127945
dc.identifier.eissn1873-7072
dc.identifier.issn0308-8146
dc.identifier.pubmed32889200
dc.identifier.urihttps://hdl.handle.net/20.500.14981/61959
dc.identifier.volume340
dc.identifier.wos000594652900003
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofFOOD CHEMISTRY
dc.subjectCobalamin
dc.subjectCobalt
dc.subjectHigh-performance liquid chromatography
dc.subjectInductively coupled plasma-optic emission spectrometry
dc.subjectKefir
dc.subjectPERFORMANCE LIQUID-CHROMATOGRAPHY
dc.subjectDAIRY-PRODUCTS
dc.subjectVITAMIN-B-12
dc.subjectEXTRACTION
dc.subjectMICROEXTRACTION
dc.subjectSAMPLES
dc.subjectNICKEL
dc.subjectMILK
dc.subjectChemistry
dc.subjectFood Science & Technology
dc.subjectNutrition & Dietetics
dc.titleA rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES
dc.typeArticle
dspace.entity.typePublication
local.import.sourceWOS

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