Radiation induced formation of poly (N-isopropyl acrylamide)-bovine serum albumin covalent conjugates and their immunogenicity

Abstract

Poly (N-isopropyl acrylamide) was bioconjugated to bovine serum albumin by Co-60 gamma-rays at pH 7. The covalent binding mechanism of the radiation induced poly (N-isopropyl acrylamide)-bovine serum albumin conjugate Was analyzed by high performance liquid chromatography while the immunization property was analyzed by ELISA tests. High performance liquid chromatography results showed the conjugate formation of polymer with protein and also the increase in the radio stability of the protein as a result of this formation. The immunization results of irradiated conjugates in Balb/c mice were significantly higher than the free BSA immunization results. This, system is attractive for application of a novel immunogenic model system in vaccine technology.