Özet:
Cycloastragenol (CAG) is a compound isolated from‘Astragalus membranaceus’ which
has been proven to significantly stimulate telomerase activity, cell proliferation and
prevent some diseases in human. Similarly, plants endure different stress conditions
leading to physiological, biochemical and molecular perturbations and affecting their
growth and development. CAG would be for plants a potential molecule preventing and
remedying these disorders. In this frame, our study, the first to investigate CAG in plants,
aims to determine effects of cycloastragenol on different signaling mechanisms, to have
an overview of transcriptional and post- transcriptional responses analysis through high
throughput sequencing methodologies, in order to reveal CAG potential help to plants in
surmounting different environmental stresses. RNA sequencing and small RNA
sequencing strategies were employed in A. thaliana roots calli under CAG treatment to
determine the transcriptional profiles, and to assess known and unknown small RNAs and
evaluate their possible functions in the affected pathways. The generated data revealed
the upregulation of different genes and stress responsive protein families such as
cytochrome P450s transporters. Besides, several miRNAs and their targets associated to
metabolic and stress tolerance signaling pathways were found to be up-regulated in
treated sample, like miR3434-5p and its target genes which interfere positively in plant
responses to hyperosmotic stresses.
